Faithful Expression of GFP from the PLC beta2 Promoter in a Functional Class of Taste Receptor Cells

摘要

Phospholipase C-type beta~2 (PLC beta~2)is expressed in a subset of cells within mammalian taste buds.This enzyme is involved in the transduction of sweet,bitter,and umami stimuli and thus is believed to be a marker for gustatory sensory receptor cells.We have developed transgenic mice expressing green fluorescent protein (GFP)under the control of the PLC beta~2 promoter to enable one to identify these cells and record their physiological activity in living preparations.Expression of GFP (especially in lines with more than one copy integrated)is strong enough to be detected in intact tissue preparations using epifluorescence microscopy.By immunohistochemistry,we confirmed that the overwhelming majority of cells expressing GFP are those that endogenously ex-press PLC beta~2.Expression of the GFP transgene in circumvallate papillae occurs at about the same time during development as endogenous PLC beta~2 expression.When loaded with a calcium-sensitive dye in situ,GFP-positive taste cells produce typical Ca~(2+)responses to a taste stimulus,the bitter compound cycloheximide.These PLC beta~2 promoter-GFP transgenic lines promise to be useful for studying taste transduction,sensory signal processing,and taste bud development.