PICHIA PASTORIS ALCOHOL OXIDASE 1 (AOX1) ISOLATED PROMOTER, VECTOR, CELL, KIT, METHOD FOR EXPRESSION OF A RECOMBINANT PROTEIN, PEPTIDE OR FUNCTIONAL NUCLEIC ACID, IN A CELL OF A CULTIC MUSCLE EXPRESSION OF A PROTEIN, PEPTIDE OR FUNCTIONAL NUCLEIC ACID, AND METHOD FOR INSULATING SUPER EXPRESSION CLONES

摘要

mutant aox1 promoters. The present invention relates to a mutant pichia pastoris oxidase 1 (aox1) promoter of the wild-type pichia pastoris aox1 promoter (seq id # 1), comprising at least one mutation selected from the group consisting of : a) a transcription factor binding site (tfbs), b) nucleotides 170 to 235 (-784 to -719), nucleotides 170 to 191 (-784 to -763), nucleotides 192 to 213 (-762 to - 741), nucleotides 192 to 210 (-762 to -744), nucleotides 207 to 209 (-747 to -745), nucleotides 214 to 235 (-740 to -719), nucleotides 304 to 350 (-650 to -604) , nucleotides 364 to 393 (-590 to -561), nucleotides 434 to 508 (-520 to -446), nucleotides 509 to 551 (-445 to -403), nucleotides 552 to 560 (-402 to -394), nucleotides 585 to 617 (-369 to -337), nucleotides 621 to 660 (-333 to -294), nucleotides 625 to 683 (-329 to -271), nucleotides 736 to 741 (-218 to -213), nucleotides 737 to 738 (-217 to -216), nucleotides 726 to 755 (-228 to -199), nucleotides 78 4 to 800 (-170 to -154) or nucleotides 823 to 861 (-131 to -93) of sequence id 1, and combinations thereof.