Cloning and characterization of two maize cDNAs encoding cinnamoyl-CoA reductase (CCR) and differential expression of the corresponding genes

摘要

Cinnamoyl-CoA reductase (CCR, EC 1.2.1.44) catalyses the first step of the lignin pathway. Two full-length cDNAs identified by sequence analysis as CCR-encoding cDNAs were isolated from a maize root cDNA library. These two cDNAs, designated ZmCCR1 and ZmCCR2, exhibit 73% sequence conservation at the nucleotide level for their coding regions and are relatively divergent at their 5'- and 3'-untranslated regions. They both contain a common signature which is thought to be involved in the catalytic site of CCR. Northern blot analysis indicated that ZmCCR2 was expressed at very low levels in roots, whereas ZmCCR1 was widely expressed in different organs. The high level of ZmCCR1 gene expression along the stalk suggests that the corresponding enzyme isprobably involved in constitutive lignification. Nucleotide sequence data have been submitted to the EMBL/GenBank/DDBJ databases under the accession numbers X98083 (ZmCCR1) and Y15069 (ZmCCR2).