A rapid and accurate strategy for rice contig map construction by combination of fingerprinting and hybridization.

摘要

A rapid and accurate strategy for rice contig map construction is described. A rice bacterial artificial chromosome (BAC) library with average inserts of 120 kb in length was used as the building material in contig mapping. Average number of redundant clones in contigs was in agreement with the degree of clone redundancy in the library. Around 600 contigs with various lengths ranging from 500 kb to several megabases were obtained, the total length of which was nearly the size of the rice genome. Contigs were then assigned to and ordered along the chromosomes by various molecular markers through their hybridization against the whole genomic library. The contig map thus constructed was integrated with the genetic map from which the genetic markers were derived, and took less than 2 years to produce.