Mutation-screening of Pleurotus ferulae with high temperature tolerance by nitrogen ion implantation

摘要

In order to obtain Pleurotus ferulae with high temperature tolerance, conidiophores of wild type strain ACK were implanted with nitrogen ions in energy of 5 similar to 15 keV and dose of 1.5x10(15) similar to 1.5 x 10(16) cm(-2), and a mutant CGMCC1763 was isolated subsequently through thermotolerant screening method. It was found that during riper period the surface layer mycelium of the mutant in mushroom bag wasn't aging neither grew tegument even above 30 degrees C. The mycelium endurable temperature of the mutant was increased by 5 degrees C compared to that of the wild type strain. The fruiting bodies growth temperature of the mutant was 18 similar to 22 degrees C in daytime and 8 similar to 14 degrees C at night. The highest growth temperature of fruiting bodies of the mutant was increased about 7 degrees C w.r.t. that of original strain. Through three generations investigations, it was found that the mutant CGMCC1763 was stable with high temperature tolerance.