Analysis of stem nodule from Sesbania rostrata using a new visualization technique of three-dimensional reconstruction

摘要

A three-dimensional (3-D) analysis technique was developed to visualize the whole nodule and Nitrogen fixation (N_2-fix) zone in a stem nodule from Sesbania rostrata infected with symbiotic rhizobium, Azorhizohium caulinodans ORS571. The reconstructed N_2-fix zone of the stem nodule was analyzed by arranging the ratio of opacity, by cutting at vertical and level direction, and by extraction in a personal computer. The N_2-fix zone was a ring shape, surrounding the vascular bundle. This is the firstreport that shows whole distribution of N_2 - fix zone in S. rostrata stem nodule.