Rapid survey for presence of a blast resistance gene Pi-ta in rice cultivars using the dominant DNA markers derived from portions of the Pi-ta gene.

摘要

The Pi-ta gene in rice confers resistance to strains of the blast pathogen Magnaporthe grisea (Herbert) Borr. (anamorph Pyricularia oryza Cav.) containing the corresponding avirulence gene AVR-Pita in a gene-for-gene fashion. The Pi-ta gene is a typical nucleotide-binding site type resistance gene. Nucleotide sequences distinguishing the resistant Pi-ta and susceptible pi-ta alleles were previously identified and used for developing DNA markers for a resistant Pi-ta haplotype and three susceptible pi-ta haplotypes. In the present study, the existence of the Pi-ta gene in 141 rice germplasm accessions was rapidly determined using these markers, and the results were confirmed by inoculating rice germplasm with an M. grisea strain containing AVR-Pita. The Pi-ta gene was found in accessions from several major rice producing countries, including China, Colombia, Japan, Vietnam, the Philippines, Iran and the United States. The usefulness of DNA markers for rapid determination of the genotype of rice germplasm was thus demonstrated. The Pi-ta gene also was found in rice cultivar known to contain the Pi-ta2 gene, although the allelic relationship of these genes remains to be determined. The presence of the Pi-ta gene in landrace cultivars in several different geographical locations, the Philippines and Vietnam, other indica rice cultivars in China and Colombia suggest that the Pi-ta gene may have spontaneously originated in indica rice cultivars. These results are useful for incorporating the Pi-ta gene into advanced breeding lines by marker-assisted selection for rice breeding programmes worldwide..