Polymorphisms and Sequencing Analysis in 5'Upstream and Intron 2 Region of H-FABP Gene in Anhui Wannan Black Pig Population

摘要

In order to give a basis for molecular breeding of meet quality traits in Wannan black pig, we applied PCR-RFLP method to analyze the polymorphism of 5'upstream region and intron 2 of H-FABP gene in 184 Wannan black pigs population. The results show that there exist polymorphic loci of restriction enzyme Hinf in 5'upstream region and Hae III as well as Hinf I in intron 2. Except for locus of restriction enzyme HinfI, the other two polymorphism sites achieved in Hardy-Weinberg equilibrium based on χ2-test (p>0.05). In addition, a T→C mutation in 1324 bp, a C→G mutation in 1811 bp and a C→T mutation in 1970 bp were found through the direct sequencing the polymorphic RFLP fragments of H-FABP gene, then genotype frequencies, gene frequencies, Ho, He, Ne and PIC were investigated in Wannan black pig population. The results identified 3 variant restriction sites and provided a basic data for further study of the relationship between IMF and H-FABP with different genotypes and also provide basic for make sure whether H-FABP gene is the key gene for effect on the deposition of IMF.