Biohydrogen production from hyperthermophilic anaerobic digestion of fruit and vegetable wastes in seawater: Simplification of the culture medium of Thermotoga maritima

摘要

Highlights•H2 production from fruit and vegetable wastes byT. maritimain seawater was studied.•The supply of seawater can replace some components necessary forT. maritima.•Using natural seawater medium recorded similar results to the mineral basal medium.•No H2production byT. maritimain absence of nitrogen and sulfur sources.Graphical abstractDisplay OmittedAbstractBiohydrogen production by the hyperthermophilic and halophilic bacteriumT. maritima, using fruit and vegetable wastes as the carbon and energy sources was studied. Batch fermentation cultures showed that the use of a culture medium containing natural seawater and fruit and vegetable wastes can replace certain components (CaCl2, MgCl2, Balch's oligo-elements, yeast extract, KH2PO4and K2HPO4) present in basal medium. However, a source of nitrogen and sulfur remained necessary for biohydrogen production. When fruit and vegetable waste collected from a wholesale market landfill was used, no decreases in total H2production (139 mmol L−1) or H2yield (3.46 mol mol−1) was observed.