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Sequence analysis of VP2 gene of canine parvovirus isolates in Thailand

机译:泰国犬细小病毒分离株VP2基因的序列分析

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Canine parvovirus (CPV) causes a very severe enteric disease especially in puppies. Twenty-six isolates of CPV were obtained from dogs at the Animal Hospital, Kasetsart University, Thailand. Whole VP2 gene of 26 isolates was amplified using polymerase chain reaction (PCR) and its sequences were analyzed. Nineteen out of 26 isolates were characterized as CPV type 2a variants and the rest of the isolates were characterized as CPV type 2b. These results indicated that both types are currently prevalent field CPV circulating in Thailand and type 2a is the predominant genotype. Neither CPV type 2 nor type 2c was observed in this study.
机译:犬细小病毒(CPV)引起非常严重的肠道疾病,尤其是在幼犬中。从泰国Kasetsart大学动物医院的狗中获得了26株CPV分离株。使用聚合酶链反应(PCR)扩增了26个分离株的完整VP2基因,并对其序列进行了分析。 26个分离株中有19个被鉴定为CPV 2a型变体,其余分离株被鉴定为CPV 2b型。这些结果表明,这两种类型都是泰国目前流行的现场CPV,而2a型是主要的基因型。在这项研究中未观察到2型CPV和2c型。

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