Microbial transformation of acetophenone to 2-phenylethanol

摘要

Mcroorganisrrs are commonly used to transform chermical to chiral compounds. Pseudomonas cepacia 1813, Pseudomonas stutzeri 1317, Saccharomyces cerevisiae 1912, and 5 Escherichia coli strains were used to transform acetophenone to 2-phenylethand. The results show that the E. coli strains have the poorest biotransformation ability. P. stutzeri 1317 provides the best transformation ability with a transforrration rate of 30% achieved with either whole or broken P. stutzeri 1317 cells for a reaction pH of 8.2 and an initial acetophenone concentration of 2g/L. An acetophenone corcentration of 10 g/L strongly inhibits the biotransformation for a pH of 8.2. Crude alcohol dehydrogenase obtained from S. cerevisiae 1912 transforms acetophenone to 2-phenyl ethand when nicotinamide adenine dinucleotide reduced (NADH) is added.