Microbial Transformation of Acetophenone to 2-Phenylethanol

摘要

Microorganisms are commonly used to transform chemicals to chiral compounds.Pseudomonas cepacia 1813, Pseudomonas stutzeri 1317, Saccharomyces cerevisiae 1912, and 5 Escherichia coli strains were used to transform acetophenone to 2-phenylethanol.The results show that the E.coli strains have the poorest biotransformation ability.P.stutzeri 1317 provides the best transformation ability with a transformation rate of 30% achieved with either whole or broken P.stutzeri 1317 cells for a reaction pH of 8.2 and an initial acetophenone concentration of 2 g/L.An acetophenone concentration of 10 g/L strongly inhibits the biotransformation for a pH of 8.2.Crude alcohol dehydrogenase obtained from S.cerevisiae 1912 transforms acetophenone to 2-phenylethanol when nicotinamide adenine dinucleotide reduced (NADH) is added.