Comparison of four methods for the extraction of lipopolysaccharide from cyanobacteria

摘要

Four procedures were compared for the extraction of lipopolysaccharide (LPS) from 10 laboratory strains of cyanobacteria. LPS yields as a percentage of cyanobacterial dry weight ranged from 0.03 to 6.13. The heterotrophic bacterial LPS marker 2-keto-3-deoxyoctonoic (KDO) acid was not detectable in any of the cyanobacterial LPS preparations. All methods yielded LPS that contained biologically- and toxicologically-significant quantities of the cyano-toxin microcystin, when applied to some, but not all, microcystin-producing cyanobacterial strains. Of the four methods used, a procedure including Proteinase K, plus RNase and DNase, performed best overall on the basis of minimum time required, higher percentage yields of LPS, and minimal co-extraction of microcystin.