ADSORPTION COMPLEXES OF COLLAGENOUS POLYPEPTIDE-IONIC SURFACTANT IN AQUEOUS MEDIUM: 1. THE FORMATION OF MICELLAR STRUCTURE OF IONIC SURFACTANT ADSORBED ONTO COLLAGENOUS POLYPEPTIDE CHAIN

摘要

The paper reports on the main findings issued from studying the formation of adsorbed micelle-like clusters of ionic surfactant (sodium dodecyl sulfate and hexadecyl trimethylammonium bromide) onto molecule of collagenous polypeptide (collagen hydrolyzates and calfskin collagen type I). Capillary viscometry, UV-circular dichroism, transmission electron microscopy and surfactant titration method were used to characterize the macromolecular component of the studied systems. To highlight the critical aggregation concentration (cac) and the critical saturation concentration (T_2) in the investigated polypeptide-surfactant systems, the steady-state fluorescence of pyrene (I1/I3 rule) and the surface tension measurements (the droplet volume method) were availed. The study revealed a significant decrease of the effective micelle concentration of surfactant (cac) in the presence of polypeptide irrespective of pH and a critical concentration T_2 depending on the system composition and pH.