Simple and Rapid Flow Injection Analysis for Determination of the Nitrite Ion in Meat and Fish Products with 1,2-Diamino-4,5-dimethoxybenzene as the Fluorogenic Reagent

摘要

A simple and rapid flow injection analysis (FIA) with fluorescence detection was developed for the determination of nitrite in meat and fish products (e.g., ham and sausages). 1,2-Diamino-4,5- dimethoxybenzene (DDB) was used as the fluorogenic reagent, and the carrier solution was 0.05 mM DDB in 0.5 M sulfuric acid which retained its availability for at least 48 h after preparation, The DDB derivative was monitored at 402 nm (ex, at 311 nm). The calibration curve for standard nitrite ranging from 0.23 to 4.60 μ g/ml showed good linearity (r= 0.998) between the concentration of nitrite and the fluorescence intensity. The limit for the detection of nitrite was 51 ng/ml at a signal-to-noise ratio of 3. The amount of nitrite in meat and fish products ranged from 7.8 to 24.1 μg/g. Good correlation (r = 0.991) was obtained between the proposed method and spectrophotometry by using a fish product spiked with a known concentration of standard nitrite.