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Cellulose degrading capabilities of celluloytic bacteria isolated from the intestinal fluids of the silver cricket

机译:从的肠液中分离出来的纤维素细菌的纤维素降解能力

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Cellulose degrading bacteria were isolated from the intestinal fluid of the silver cricket Lepisma sp. and a culture was developed anoxigenically in the cellulose degrading medium following standard laboratory techniques. The celluloytic activity of the microbe was examined in a broth culture using Whatman 42 filter paper as the soruce of insoluble cellulose. Activity was measured spectrophotometrically (620nm) following Anthrone reaction of the culture filtrate and the sugawr produced was quantified as a factor of time. The pH optimum was found to be between 7 and 8. Temoperature profile optimum was between 30-37 deg C.
机译:从银Lepisma sp。的肠液中分离出降解纤维素的细菌。然后按照标准实验室技术,在纤维素降解培养基中厌氧培养培养物。使用Whatman 42滤纸作为不溶性纤维素的来源,在肉汤培养物中检查微生物的纤维素活性。在培养物滤液的蒽酮反应后,用分光光度法(620nm)测量活性,并将产生的糖蜜定量化为时间因素。发现最适pH为7至8。最佳温度为30-37℃。

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