USING FLOW CYTOMETRY TO MEASURE SIGNALING BY PERIPHERAL BLOOD LEUKOCYTES THROUGH CD11B/CD18 RECEPTORS DURING SEPSIS IN A PORCINE MODEL

摘要

The systemic inflammatory response associated with traumatic injuries and sepsis can result in disruption of vascular permeability and subsequent pathophysiological derangements that may contribute to the development of abdominal compartment syndrome, multiple organ failure, and death. The ability to detect potentially lethal progression of inflammation following injury and sepsis is of paramount clinical importance. Activation of polymorphonuclear cells (PMNs) resulting in expression of surface adhesion markers (CDllb and CD18) and subsequent activation are intricately involved in injury related inflammation. We hypothesize that ascites is capable of activating naive neutrophils (NEU) and that measuring the NEU priming capability of ascites could be a useful way to monitor progression of systemic inflammation and treatment effectiveness.