DEFFERENCES IN SEQUENCES OF RIBOSOMAL DNA SECOND INTERNAL TRANSCRIBED SPACER AMONG THREE MEMBERS OF ANOPHELES HYRCANUS COM- PLEX FROM THE REPUBLIC OF KOREA

摘要

Differences in sequences of ribosomal DNA second internal transcribed spacer (ITS2) among Anopheles sinensis, An. Lesteri and An. Yatsushiroensis from Korea were compared. The PCR amplified RDNA-ITS2 fragments were sequenced directly. Three samples of each species were individually determined. Lengths of the ITS2 regions were 468bp in An. Sinensis, 451bp in An. Lesteri and 453bp in An. Yatsushi- Roenesis and GC contents were 44.87/100, 46.2/100 and 45.7/100 respectively. Variations of the sequences ranged From 12.16/100 to 30.7/100 among the three species. The differences o the rDNA-ITS2 sequences would be useful For molecular identification of the three members of Anopheles hyrcanus comples from Korea.