Fabrication of capillary force induced DNA template Ag nanopatterns for sensitive and selective enzyme-free glucose sensors

摘要

Graphical abstractDisplay OmittedHighlights•Selfpatterned Ag nanowires on ITO were developed using DNA sacrificial template.•Mechanism involved in Ag nanowires formation is detailed.•Influences of morphological features toward glucose electrooxidation are detailed.•Active metal sites involved enzyme free glucose detection is illustrated.•Real sample analysis in human blood was achieved with the fabricated sensors.AbstractThe self-patterned silver (Ag) nanowires on Indium Tin Oxide (ITO) were developed and exploited as the enzyme-free sensor probes for glucose detection. Deoxyribonucleic acid (DNA) template increased the capillary force channels of Ag and facilitated the homogeneous and high mobility of Ag-DNA toward the scribeviathe coffee ring effect. The subsequent removal of DNA template from Ag-DNA/ITO through the enzymatic hydrolysis process led to the formation of Ag nanowires on ITO. Under alkaline conditions, Ag nanopatterns developed on ITO realized the considerable enzyme-free glucose sensor performances. The fabricated sensor system is reproducible and stable and was pertained with an analysis of spiked human blood serum, where it provided excellent recoveries. Thus these findings have not only showered insights on the self assembly of Ag nanoparticles without the exploitation of any surfactants and harsh conditions but have also provided the fundamental perceptive on the influences of self-assembled nanowires in enzyme free glucose sensor applications.