Abundance and community succession of nitrogen-fixing bacteria in ferrihydrite enriched cultures of paddy soils is closely related to Fe(Ⅲ)-reduction

摘要

In flooded paddy soils, some metal reducers are also capable of nitrogen (N) fixation, which is essential in ensuring a reliable N-supply for rice growth. Microbial iron [Fe(Ⅲ)] reduction is an important biogeochemical process that can be stimulated by ferrihydrite amendment to paddy soil. Therefore, this study aimed to investigate the abundance and succession of the N_2-fixing bacterial community in ferrihydrite enriched paddy soils collected from Hunan (HN) and Sichuan (SC) provinces, China. The relationship between the N_2-fixing bacterial community and Fe(Ⅲ) reduction was also assessed. When compared with the control treatment, ferrihydrite enrichment significantly enhanced nitrogenase (nifH) gene abundance by 8.05 × 10~5 to 4.45 × 10~6 copies g~(-1) soil during the 40-day flooding of HN soil, while nifH gene abundance in SC soil was remarkably increased by 5.90 × 10~7 to 9.56 × 10~7 copies g~(-1) soil during day 1 to 5 in response to ferrihydrite amendment. The relative abundance of N_2-fixing bacteria peaked on day 5 (21.5% in HN soil and 5.4% in SC soil) and gradually decreased to a stable abundance after day 20. Remarkable increases in relative abundance of N_2-fixing bacteria during the first 10 days of flooding were detected in both soils with ferrihydrite enrichment, whereas little difference was found after day 10 of flooding. During the early stage of flooding, the Shannon and Simpson indexes of N_2-fixing bacteria with ferrihydrite enrichment were significantly decreased, and the community structure changed greatly. Most N_2-fixing bacteria in ferrihydrite enriched paddy soils were phylogenetically related to the order Clostridiales, with some of those potentially capable of Fe(Ⅲ) reduction. The community succession of N_2-fixing bacteria closely correlated with Fe(Ⅲ) reduction. Thus, improving N_2-fixation via stimulation of Fe(Ⅲ) reduction might aid in the reduction of N-fertilizer application to paddy field.