Insulin precursors

摘要

Methods have been described to produce human insulin analogues and precursors thereof in genetically modified yeasts (Kjeldsen T. Applied Microbiology and Biotechnology 2000, 54. 277-286). One important parameter to obtain high yields of cxprcsscd and secreted precursors from the yeast is the composition and sequence of the C-peptide inserted between the B-chain and the A-chain (B-C-A). This disclosure lists ten he xamer amino acid C-peptides that have been found to improve expression yields of insulin precursor analogues having a truncated B-chain without B29 and B30 (DesB29-30) compared to precursors not having a C peptide.