Polymorphism of HIV-1 Resistance Genes in Dai and Jingpo Minorities of Yunnan Province

摘要

Objective To investigate the mutant frequency and polymorphism of HIV-1 resistance CCR5-Δ32, CCR2-64I, SDF1-3'A alleles in Jingpo and Dai nationalities of Yunnan Methods The study population included 101 Dai and 113 Jingpo ethnical subjects. The genotypes were respectively detected by polymerase chain reaction (PCR) or by PCR/RFLP (restriction fragment length polymorphism) assay. Mutant frequencies were calculated and allelic polymorphism of the three genes in population was analyzed by U test. Results We didn't find CCR5- Δ32 mutant in either Dai or Jingpo nationality. In Dai minority, the allele frequency of CCR2-64I was 21.00% and that of SDFJ-3'A was 20.30%. In Jingpo minority, the allele frequency of CCR2-64I was 16.37% and that of SDF1 -3'A was 17.70%. Conclusion Dai and Jingpo nationality of Yunnan might have a high genetic susceptibility to HIV-1 (including R5 and X4 HIV strains) since we didn't find CCR5-Δ32 and the frequency of SDF1-3'A was much lower than that of Han nationality as was reported in other research papers.