The Use of Isothermal Titration Calorimetry in Drug Design: Applications to High Affinity Binding and Protonation/Deprotonation Coupling

摘要

Isothermal Titration Calorimetry (ITC) plays a central role in the characterization and optimization of lead compounds as viable drug candidates. ITC is the only technique that permits a complete experimental characterization of the binding affinity of a ligand in terms of its thermodynamic components ΔG, ΔH, ΔS and ΔCp. In addition, ITC is ideally suited to characterize coupled processes like protonation/deprotonation reactions.