首页> 外文期刊>Renewable energy >Towards sequential bioethanol and L-lactic acid co-generation: Improving xylose conversion to L-lactic acid in presence of lignocellulosic ethanol with an evolved Bacillus coagulans
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Towards sequential bioethanol and L-lactic acid co-generation: Improving xylose conversion to L-lactic acid in presence of lignocellulosic ethanol with an evolved Bacillus coagulans

机译:朝向顺序生物乙醇和L-乳酸生成:在具有进化的芽孢杆菌的木质纤维素乙醇存在下将木糖转化改善为L-乳酸

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摘要

A wide range of biofuels and bio-based products can be produced from lignocellulose considering its high compositional diversity. Ethanol production by yeasts from cellulosic glucose is well-known, while hemicellulosic xylose utilization is still challenging. This work proposes the use the xylose for L-lactic acid fermentation. In this context, a sequential cultivation of Saccharomyces cerevisiae and the C5-utilizing Bacillus coagulans is studied. High ethanol yields, around 0.44 g g(-1), were obtained from a cellulosic-gardening hydrolysate. The high ethanol concentrations did not affect the evolved B. coagulans A20-EXA obtained by adaptive evolution to ethanol. As a result, 2.6-fold increase in lactic acid yield was achieved when compared with parental B. coagulans strain in presence of 5% (v v(-1)) ethanol. These results demonstrated the suitability of B. coagulans A20-EXA to be used together with S. cerevisiae for the sequential co-generation of ethanol and lactic acid from lignocellulosic biomass in a biorefinery approach. (C) 2020 Elsevier Ltd. All rights reserved.
机译:考虑到其高分式化多样性,可以从木质纤维素生产各种生物燃料和生物燃料。来自纤维素葡萄糖的酵母产生的乙醇是众所周知的,而半纤维素木糖利用仍在具有挑战性。这项工作提出了使用木糖用于L-乳酸发酵。在这种情况下,研究了酿酒酵母的连续培养和利用芽孢杆菌的C5和C5。从纤维素 - 园艺水解产物获得大约0.44gg(-1)的高乙醇产率。高乙醇浓度不影响通过适应性进化与乙醇获得的进化B.凝固的B.凝固性A20-EXA。结果,与亲本B的凝壳菌株在5%(V(-1))乙醇的存在下,达到2.6倍的乳酸产量增加。这些结果证明了B. CoAGulans A20-EXA的适用性与S. Cerevisiae一起使用,以便在生物颗粒方法中与木质纤维素生物量的连续生成乙醇和乳酸。 (c)2020 elestvier有限公司保留所有权利。

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