USE OF A DUAL-LABELLED OLIGONUCLEOTIDE AS A DNA DOSEMETER FOR RADIOLOGICAL EXPOSURE DETECTION

摘要

A reporter molecule consisting of a synthetic oiigonucleotide is being characterised for a novel damage detection scenario for its potential use as a tield-deplovable, personal deoxyribonucleic acid (DNA) dosemeter for radiation detection. This dose-meter is devoid of any biological properties other than being naked DNA and therefore has no DNA repair capabilities. It supports biodosimetry techniques, which require lengthy analysis of cells from irradiated individuals, and improves upon inorganic dosimetry, thereby providing for a more relevant means of measuring the accumulated dose from a potentially mixed-radiation field. Radiation-induced single strand breaks (SSBs) within the DNA result in a quantifiable fluorescent signal. Proof of concept has been achieved over 250 mGy-10 Gy dose range in radiation fields from ~(60)Co, with similar results seen using a linear accelerator X-ray source. Further refinements to both the molecule and the exposure/detection platform are expected to lead to enhanced levels of detection for mixed-field radiological events.