Solid state production of thermostable pectinases from thermophilic Thermoascus aurantiacus

摘要

Pectin lyase (Pl) and polygalacturonase (Pg) production by Thermoascus aurantiacus 179-5 was carried out by means of solid-state fermentation using orange bagasse, sugar cane bagasse and wheat bran as a carbon sources. Pg and Pl had optimum activity at pH 5.0 and 10.5-11.0, respectively. Maximal activity of the enzymes were determined at 65 deg. C. Pg was stable in the acidic to neutral pH range and at 60 deg. C for 1 h, whereas Pl was stable at acidic pH and at 60 de.g C for 5 h.