Expression in yeast of binding regions of karyopherins α and β inhibits nuclear import and cell growth

摘要

Using truncated forms of recombinant yeast karyopherinsα and β in in vitro binding assays, we mapped the regions of karyopherin α that bind to karyopherin β and the regions of karyopherin βthat interact with karyopherin αand with Ran-GTP. Karyopherin α's binding region for karyopherin β was localized to its N-terminal domain, which contains several clusters of basic residues, whereas karyo- pherin β's binding region for karyopherin α was localized to an internal region containing two clusters of acidic residues.