Transcription factor TFIIH and DNA endonuclease Rad2 constitute yeast nucleotide excision repair factor 3: Implications for nucleotide excision repair and Cockayne syndrome

摘要

Nucleotide excision repair (NER) of ultravi- olet light-damaged DNA in eukaryotes requires a large num- ber of highly conserved protein factors. Recent studies in yeast have suggested that NER involves the action of distinct protein subassemblies at the damage site rather than the placement there of a "preformed repairosome" containing all the essen- tial NER factors. Neither of the two endonucleases, Rad1- Rad10 and Rad2, required for dual incision, shows any affinity for ultraviolet-damaged DNA.