Cibacron Blue F3GA was covalently attached onto magnetic poly(vinyl alcohol) (mPVAL) beads (100-150 μm in diameter) for human serum albumin (HSA) adsorption from human plasma. Despite low nonspecific adsorption of HSA on mPVAL beads, Cibacron Blue F3GA attachment significantly increased the HSA adsorption. The maximum HSA adsorption was observed at pH 5.0. Higher HSA adsorption was observed from human plasma. Desorption of HSA from mPVAL beads was achieved by medium containing 1.0 M KSCN at pH 8.0. To test the efficiency of albumin adsorption from human serum, before and after albumin adsorption was demonstrated with sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analyses. HSA molecules could be reversibly adsorbed and desorbed 10 times with the magnetic beads without noticeable loss in their HSA adsorption capacity.View full textDownload full textKeywordsaffinity beads, albumin adsorption, Cibacron Blue F3GA, dye ligands, magnetic beads, PVALRelated var addthis_config = { ui_cobrand: "Taylor & Francis Online", services_compact: "citeulike,netvibes,twitter,technorati,delicious,linkedin,facebook,stumbleupon,digg,google,more", pubid: "ra-4dff56cd6bb1830b" }; Add to shortlist Link Permalink http://dx.doi.org/10.1080/10826068.2010.540606
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机译：烟草蓝F3GA被共价结合到磁性聚乙烯醇（mPVAL）珠（直径100-150μm），用于从人血浆中吸附人血清白蛋白（HSA）。尽管在mPVAL磁珠上HSA的非特异性吸附较低，但是Cibacron Blue F3GA附着显着增加了HSA吸附。在pH 5.0时观察到最大的HSA吸附。从人血浆中观察到更高的HSA吸附。从mPVAL珠的HSA的解吸是通过包含1.0 pH 8.0的1.0 KSCN的介质实现的。为了测试从人血清中吸附白蛋白的效率，通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）分析证明了白蛋白吸附前后。 HSA分子可以用磁珠可逆地吸附和解吸10次，而不会明显降低HSA吸附能力。查看全文下载全文关键词亲和珠，白蛋白吸附，Cibacron Blue F3GA，染料配体，磁珠，PVAL相关变量add addthis_config = {ui_cobrand： “泰勒和弗朗西斯在线”，services_compact：“ citeulike，netvibes，twitter，technorati，delicious，linkedin，facebook，stumbleupon，digg，google，更多”，发布：“ ra-4dff56cd6bb1830b”};添加到候选列表链接永久链接http://dx.doi.org/10.1080/10826068.2010.540606
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