Callus formation and plant regeneration from protoplasts of sunflower calli and hypocotyls

摘要

Sunfower(cv. Girapac SH222) protoplasts were obtained from 4-7 day-old hypocotyls and cotyledons and from two-month old calli. Higher yields of protoplasts were achieved with medium E1(DC1 25 g.dm~-3, CaC1_2 2 g. dm~-3, MES 0.7 g.dm~-3,pH 5.5) and the combination of Driselase fluka 0.2/100, Macerozyme Onozuka 0.2/100 and Cellulase Onozuka R10 0.2/100. Hypocotyls gave the highest of protoplasts, followed by cotyledons and calli. Protoplasts Were cultivated on liquid and on solid media using both L4M(Burrus et al.,1991) and V-KM (Bokelman and Roest, 1983) media.