Plasma-Based Modification of Polystyrene Surfaces for Serum-Free Culture of Osteoblastic Cell Lines

摘要

Cell culture based diagnostics are of crucial interest for the investigation of osteoporosis, as well as for the screening of new drugs for their corresponding curativeness. In these applications, it is important to cultivate bone cells (HOS 58 osteosarcoma) permanently in a differentiation permitting state in a serum-free environment. However, the adhesion of HOS 58 cells on polystyrene surfaces takes place in a poor and insufficiently reproducible manner. Thus, the aim of this study was the improvement of polystyrene surface properties to support such a screening system utilizing HOS 58 cells. An ammonia microwave plasma-based surface modification was developed. Cell culture substrates were equipped with a high density of amino groups, which were utilized for the covalent coupling of collagen Ⅰ. Thus, the extent and observed homogeneity of HOS 58 cell adhesion were improved markedly. This suggests the possibility of controlling adherence, growth and differentiation of osteoblastic cells desired on a surface for in vitro use.