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>A Missense Mutation in the Arabidopsis COPII Coat Protein Sec24A Induces the Formation of Clusters of the Endoplasmic Reticulum and Golgi Apparatus
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A Missense Mutation in the Arabidopsis COPII Coat Protein Sec24A Induces the Formation of Clusters of the Endoplasmic Reticulum and Golgi Apparatus
n nnnHow the endoplasmic reticulum (ER) and the Golgi apparatus maintaintheir morphological and functional identity while working inconcert to ensure the production of biomolecules necessary forthe cell's survival is a fundamental question in plant biology.Here, we isolated and characterized an Arabidopsis thalianamutant that partially accumulates Golgi membrane markers anda soluble secretory marker in globular structures composed ofa mass of convoluted ER tubules that maintain a connection withthe bulk ER. We established that the aberrant phenotype wasdue to a missense recessive mutation in sec24A, one of the threeArabidopsis isoforms encoding the coat protomer complex II (COPII)protein Sec24, and that the mutation affects the distributionof this critical component at ER export sites. By contrast,total loss of sec24A function was lethal, suggesting that Arabidopsissec24A is an essential gene. These results produce importantinsights into the functional diversification of plant COPIIcoat components and the role of these proteins in maintainingthe dynamic identity of organelles of the early plant secretorypathway.展开▼
机译：ABSTRACTn n
n TOP n <字体颜色= 464c53>抽象 n 介绍 n 结果 n 讨论 n 方法 n 参考文献 n n nnn内质网（ER）和高尔基体如何维持 它们在 音乐会上工作时的形态和功能同一性，以确保产生 细胞生存所必需的生物分子，这是植物生物学中的一个基本问题。 这里，我们分离并鉴定了拟南芥 突变体，该突变体在由 <组成的球状结构中部分积累了高尔基体膜标志物和 可溶性分泌标志物/ SUP>大量曲折的ER小管，它们与 散装ER保持连接。我们确定异常表型为 ，这是由于 sec24A 的错义隐性突变所致，这是拟南芥的三个同工型之一编码外套原复合体II（COPII） 蛋白Sec24，并且该突变影响该关键成分在ER出口位点的分布 。相反， sec24A 功能的全部 致死性是致命的，这表明拟南芥 sec24A 是必需基因。这些结果对植物COPII 外壳成分的功能多样化以及这些蛋白质在维持 细胞器动态特性中的作用产生了重要的见解。早期植物分泌 途径。展开▼
