n nnnZEP1, a transverse filament (TF) protein, is the rice (Oryzasativa) homolog of Arabidopsis thaliana ZYP1. In the Tos17-insertionalzep1 mutants, homologous chromosomes align along the entirelength of the chromosome, but the synaptonemal complex is notassembled in early prophase I. Crossovers are well formed, and12 bivalents could be detected from diakinesis to metaphaseI, which leads to equal chromosomal segregation in anaphaseI. Moreover, the number of crossovers has a tendency to be increasedcompared with that in the wild type. These phenomena are differentfrom the TF mutants identified so far in other organisms. Chiasmaterminalization of the bivalent, which occurs frequently inthe wild type, seldom occurred in zep1. Transmission electronmicrographs and immunodetection using an antibody against ZEP1showed that ZEP1 is the central element of the synaptonemalcomplex. Although PAIR2 and MER3 were loaded normally in zep1,their dissociation was delayed severely compared with the wildtype. In addition, ZEP1 is reloaded onto chromosomes in earlymicrospores as the chromosome decondense, suggesting that ZEP1might have other biological functions during this process.展开▼
机译:ABSTRACTn FONT> TH> TR> TABLE> n
n TOP n <字体颜色= 464c53>抽象 FONT> n 介绍 n 结果 n 讨论 n 方法 n 参考文献 n FONT> TH> TR> TABLE> n nnnZEP1是一种横丝(TF)蛋白,是水稻( Oryza SUP> sati va I>)拟南芥 I> ZYP1的同源物。在 Tos17 I> -insertional SUP> zep1 I>突变体中,同源染色体沿染色体的整个 SUP>长度排列,但在突触中排列复杂的不是在前期I早期组装的 SUP>。交叉形成得很好,并且可以检测到从诊断运动到中期 SUP> I的 SUP> 12二价,这导致 SUP> I后期进行等价染色体分离。而且,与野生型相比,交叉的数量有增加的趋势 SUP>。这些现象与迄今为止在其他生物中鉴定出的TF突变体不同。二价体的Chiasma SUP>末端化在野生型 SUP>中经常发生,而在 zep1 I>中很少发生。透射电子显微镜照片和使用ZEP1 SUP>抗体的免疫检测结果表明,ZEP1是突触 SUP>复合体的核心元素。尽管PAIR2和MER3通常以 zep1 I>装载,但 SUP>与野生型 SUP>相比,它们的解离被严重延迟。此外,随着染色体的解聚,ZEP1会重新加载到早期 SUP>早期小孢子的染色体上,这表明ZEP1 SUP>在此过程中可能还具有其他生物学功能。 SUP>
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State Key Laboratory of Plant Genomics and Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China;
State Key Laboratory of Plant Genomics and Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China;
State Key Laboratory of Plant Genomics and Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China;
Key Laboratory of Crop Genetics and Physiology of Jiangsu Province/Key Laboratory of Plant Functional Genomics of Ministry of Education, Yangzhou University, Yangzhou 225009, China;
State Key Laboratory of Plant Genomics and Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China;
State Key Laboratory of Plant Genomics and Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China;
Key Laboratory of Crop Genetics and Physiology of Jiangsu Province/Key Laboratory of Plant Functional Genomics of Ministry of Education, Yangzhou University, Yangzhou 225009, China;
Key Laboratory of Crop Genetics and Physiology of Jiangsu Province/Key Laboratory of Plant Functional Genomics of Ministry of Education, Yangzhou University, Yangzhou 225009, China;
State Key Laboratory of Plant Genomics and Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China;