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>Arabidopsis RNA-Dependent RNA Polymerases and Dicer-Like Proteins in Antiviral Defense and Small Interfering RNA Biogenesis during Turnip Mosaic Virus Infection
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Arabidopsis RNA-Dependent RNA Polymerases and Dicer-Like Proteins in Antiviral Defense and Small Interfering RNA Biogenesis during Turnip Mosaic Virus Infection
n nnnPlants respond to virus infections by activation of RNA-basedsilencing, which limits infection at both the single-cell andsystem levels. Viruses encode RNA silencing suppressor proteinsthat interfere with this response. Wild-type Arabidopsis thalianais immune to silencing suppressor (HC-Pro)-deficient Turnipmosaic virus, but immunity was lost in the absence of DICER-LIKEproteins DCL4 and DCL2. Systematic analysis of susceptibilityand small RNA formation in Arabidopsis mutants lacking combinationsof RNA-dependent RNA polymerase (RDR) and DCL proteins revealedthat the vast majority of virus-derived small interfering RNAs(siRNAs) were dependent on DCL4 and RDR1, although full antiviraldefense also required DCL2 and RDR6. Among the DCLs, DCL4 wassufficient for antiviral silencing in inoculated leaves, butDCL2 and DCL4 were both involved in silencing in systemic tissues(inflorescences). Basal levels of antiviral RNA silencing andsiRNA biogenesis were detected in mutants lacking RDR1, RDR2,and RDR6, indicating an alternate route to form double-strandedRNA that does not depend on the three previously characterizedRDR proteins.展开▼
机译：ABSTRACTn n
n TOP n <字体颜色= 464c53>抽象 n 介绍 n 结果 n 讨论 n 方法 n 参考文献 n n nnn植物通过激活基于RNA的 silenc对病毒感染做出反应ing，这限制了单细胞和 系统级别的感染。病毒编码干扰该反应的RNA沉默抑制蛋白 。野生型拟南芥 对缺乏沉默抑制因子（HC-Pro）的 Turnip 花叶病毒免疫，但是在缺少DICER-LIKE 蛋白DCL4和DCL2的情况下，免疫力丧失。对缺乏依赖RNA的RNA聚合酶（RDR）和DCL蛋白的组合 的拟南芥突变体的敏感性 和小RNA形成的系统分析揭示了 ，绝大多数病毒衍生的小干扰RNA （siRNA）都依赖DCL4和RDR1，尽管完整的抗病毒 防御也需要DCL2和RDR6。在DCL中，DCL4足以 抵抗接种叶片中的抗病毒沉默，但是 DCL2和DCL4都参与了全身组织的沉默 （花序） 。在缺少RDR1，RDR2， 和RDR6的突变体中检测到基础水平的抗病毒RNA沉默和 siRNA生物发生，这表明形成双链 不依赖于先前鉴定的三个 RDR蛋白的RNA。展开▼
