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外文期刊>THE PLANT CELL
>HSP70 and Its Cochaperone CPIP Promote Potyvirus Infection in Nicotiana benthamiana by Regulating Viral Coat Protein Functions
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HSP70 and Its Cochaperone CPIP Promote Potyvirus Infection in Nicotiana benthamiana by Regulating Viral Coat Protein Functions
n nnnThis study demonstrates that heat shock protein 70 (HSP70) togetherwith its cochaperone CPIP regulates the function of a potyviralcoat protein (CP), which in turn can interfere with viral geneexpression. HSP70 was copurified as a component of a membrane-associatedviral ribonucleoprotein complex from Potato virus A–infectedplants. Downregulation of HSP70 caused a CP-mediated defectassociated with replication. When PVA CP was expressed in trans,it interfered with viral gene expression and replication-associatedtranslation (RAT). However, CP produced in cis interfered specificallywith RAT. CPIP binds to potyviral CP, and overexpression ofCPIP was sufficient to restore RAT inhibited by expression ofCP in trans. Restoration of RAT was dependent on the abilityof CPIP to interact with HSP70 since expression of a J-domainmutant, CPIP66, had only a minor effect on RAT. CPIP-mediateddelivery of CP to HSP70 promoted CP degradation by increasingits ubiquitination when assayed in the absence of virus infection.In conclusion, CPIP and HSP70 are crucial components of a distincttranslation activity that is associated with potyvirus replication.展开▼
机译:ABSTRACTn FONT> TH> TR> TABLE> n
n TOP n <字体颜色= 464c53>抽象 FONT> n 介绍 n 结果 n 讨论 n 方法 n 参考文献 n FONT> TH> TR> TABLE> n nnn这项研究证明热休克蛋白70(HSP70)一起 SUP> CPIP及其伴侣蛋白CPIP调节potyviral SUP>外壳蛋白(CP)的功能,而该蛋白又会干扰病毒基因 SUP>的表达。 HSP70被纯化为来自马铃薯A病毒感染的 SUP>植物的膜相关 SUP>病毒核糖核蛋白复合物的成分。 HSP70 I>的下调导致了CP介导的缺陷 SUP>与复制有关。当PVA CP反式表达时, SUP>会干扰病毒基因表达和与复制相关的 SUP>翻译(RAT)。但是,顺式产生的CP特异性地干扰了RAT。 CPIP与杯状病毒CP结合, SUP> CPIP的过表达足以恢复被 SUP> CP反式表达抑制的RAT。由于J结构域 SUP>突变体CPIP SUP> .gif“ ALT =” {Delta}“ BORDER =” 0“> 66 SUP>对RAT的影响很小。在没有病毒感染的情况下进行测定时,CPIP介导的 SUP>向HSP70的CP递送通过增加 SUP>泛素化来促进CP降解。 HSP70是与杯状病毒复制相关的独特 SUP>翻译活动的重要组成部分。 SUP>
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