A Specific Picomolar Hybridization-Based ELISA Assay for the Determination of Phosphorothioate Oligonucleotides in Plasma and Cellular Matrices

Xiaohui Wei; Guowei Dai; Guido Marcucci; Zhongfa Liu; Dale Hoyt; William Blum; Kenneth K. Chan

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A Specific Picomolar Hybridization-Based ELISA Assay for the Determination of Phosphorothioate Oligonucleotides in Plasma and Cellular Matrices

机译：一种基于皮摩尔杂交的ELISA方法，用于测定血浆和细胞基质中的硫代磷酸酯寡核苷酸

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摘要

To develop and validate an ultrasensitive and specific hybridization-based enzyme-linked immunosorbent assay method for quantification of two phosphorothioate oligonucleotides (PS ODNs) (G3139 and GTI-2040) in biological fluids.

机译：开发和验证一种基于超灵敏和特异性杂交的酶联免疫吸附测定方法，用于定量生物液体中的两个硫代磷酸酯寡核苷酸（PS ODN）（G3139和GTI-2040）。

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来源
《Pharmaceutical Research》 |2006年第6期|1251-1264|共14页
作者
Xiaohui Wei; Guowei Dai; Guido Marcucci; Zhongfa Liu; Dale Hoyt; William Blum; Kenneth K. Chan;
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作者单位

Division of Pharmaceutics College of Pharmacy The Ohio State University Columbus Ohio 43210 USA;

Division of Pharmaceutics College of Pharmacy The Ohio State University Columbus Ohio 43210 USA;

The Comprehensive Cancer Center Rm 308 OSU CCC The Ohio State University 410 West 12th Ave Columbus Ohio 43210 USA;

Division of Pharmaceutics College of Pharmacy The Ohio State University Columbus Ohio 43210 USA;

Division of Pharmacology College of Pharmacy The Ohio State University Columbus Ohio 43210 USA;

Division of Hematology-Oncology College of Medicine and Public Health The Ohio State University Columbus Ohio 43210 USA;

Division of Pharmaceutics College of Pharmacy The Ohio State University Columbus Ohio 43210 USA;

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正文语种 eng
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关键词
hybridization–ligation ELISA; intracellular drug level; pharmacokinetics; phosphorothioate oligonucleotides;

机译：杂交-连接ELISA;细胞内药物水平;药代动力学;硫代磷酸酯寡核苷酸;

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专利

1. A specific picomolar hybridization-based ELISA assay for the determination of phosphorothioate oligonucleotides in plasma and cellular matrices. [J] . Wei X, Dai G, Marcucci G, Pharmaceutical research . 2006,第6期

机译：一种基于皮摩尔杂交的特定ELISA分析法，用于测定血浆和细胞基质中的硫代磷酸酯寡核苷酸。
2. A COMPETITIVE ENZYME HYBRIDIZATION ASSAY FOR PLASMA DETERMINATION OF PHOSPHODIESTER AND PHOSPHOROTHIOATE ANTISENSE OLIGONUCLEOTIDES [J] . Jean-Robert Deverre, Valerie Boutet, Didier Boquet, Nucleic Acids Research . 1997,第18期

机译：竞争性酶加氢法测定血浆中的磷酸酯和磷酸根反义寡核苷酸
3. A competitive enzyme hybridization assay for plasma determination of phosphodiester and phosphorothioate antisense oligonucleotides [J] . Didier Boquet, Eric Ezan, Jacques Grassi, Nucleic acids research . 1997,第18期

机译：用于血浆中磷酸二酯和硫代磷酸酯反义寡核苷酸测定的竞争性酶杂交试验
4. Determination of a phosphorothioate oligonucleotide and its 3'n-1 metabolite by LC-MS from rat plasma [C] . Buyun Chen, Michael G. Bartlett American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2010

机译：用大鼠等离子体测定LC-MS硫代磷酸寡核苷酸及其3'N-1代谢物
5. Determination of two short-lived radioisotopes (radium-226, lead-210) by inductively coupled plasma mass spectrometry (ICP-MS) in environmental matrices. [D] . Lariviere, Dominic. 2005

机译：通过电感耦合等离子体质谱法（ICP-MS）在环境基质中测定两种短寿命放射性同位素（放射性226，铅210）。
6. A competitive enzyme hybridization assay for plasma determination of phosphodiester and phosphorothioate antisense oligonucleotides. [O] . J R Deverre, V Boutet, D Boquet, 1997

机译：一种用于血浆测定磷酸二酯和硫代磷酸酯反义寡核苷酸的竞争性酶杂交测定法。
7. A competitive enzyme hybridization assay for plasma determination of phosphodiester and phosphorothioate antisense oligonucleotides. [O] . Deverre, J R, Boutet, V, Boquet, D, 1997

机译：一种用于血浆测定磷酸二酯和硫代磷酸酯反义寡核苷酸的竞争性酶杂交测定法。

A Specific Picomolar Hybridization-Based ELISA Assay for the Determination of Phosphorothioate Oligonucleotides in Plasma and Cellular Matrices

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