A cholic acid-based fluorescent chemosenor for the detection of ATP

摘要

A novel ditopic cholic acid-based fluorescent chemosensor for ATP, la, was designed and synthesized. Its interactions with phosphates, AMP, ADP, ATP, CTP, GTP, and TTP have been investigated. When ATP was added to a 1 : 1 aqueous CH_3CN solution of the sensor at pH 7.4, a significant decrease in fluorescence of la was observed, whereas other guest molecules showed a much smaller effect. The complex between la and ATP was confirmed through combined UV, ~1H, ~(13)C and ~(31)PNMR spectroscopic methods. The uniqueness of the new sensor is that it binds with ATP 33-124 times more selectively than other nucleotides, as evidenced from the respective binding constants, la is a highly sensitive sensing probe; as little as 30 nM ATP can cause 15% fluorescence quenching of the sensor.