A SUPRAMOLECULAR FLUORESCENT MARKER FOR THE DETECTION AND QUANTIFICATIONOF GTP AND ATP IN BUFFER AND BIOLOGICAL FLUIDS

摘要

The present invention provides a supramolecular fluorescent marker comprising of a cyclic conjugate containing two aryl groups, one of which is anthracene, linked together by suitable spacer moieties, represented by Formula 1 and highly fluorescent indicator HPTS, for the detection and quantification of GTP and ATP under physiological pH conditions and in biological fluids. This supramolecular marker is highly soluble in aqueous medium, extremely stable in the dark and under irradiation conditions and exhibit favorable absorption and fluorescence emission properties. The cyclic conjugate of Formula 1 forms an effective complex with the highly fluorescent indicator; HPTS resulting in the formation of a supramolecular marker. The supramolecular marker thus formed is found to be effective in differentiating selectively GTP and ATP from other nucleotides and recognizes GTP and ATP through "turn-on" fluorescence mechanism. Accordingly, the supramolecular marker comprising of cyclic conjugate with the general Formula 1 and the fluorescent indicator, HPTS, is extremely useful as a visual fluorescent marker for the detection and quantification of GTP and ATP under physiological pH conditions and in biological fluids.