Identification of carbohydrate anomers using ion mobility-massn spectrometry

摘要

Carbohydrates are ubiquitous biological polymers that are important in a broad range of biological processes(1-3). However, owing to their branched structures and the presence of stereogenic centres at each glycosidic linkage between monomers, carbohydrates are harder to characterize than are peptides and oligonucleotides(4). Methods such as nuclear magnetic resonance spectroscopy can be used to characterize glycosidic linkages, but this technique requires milligram amounts of material and canno