Detection of clonal B cells in microdissected reactive lymphoproliferations: possible diagnostic pitfalls in PCR analysis of immunoglobulin heavy chain gene rearrangement

摘要

Aims-To evaluate the specificity of standard and fluorescence based (GENES- CAN) polymerase chain reaction (PCR) Immunoglobulin heavy chain (IgH) gene Rearrangement analysis in complete and Microdissected paraffin was embedded Sections from lymphoid proliferations. Methods-PCR IgH gene rearrangement Analysis of whole sections and microdis- Sected fragments (n=62) from paraffin Wax embedded reactive lymph nodes (n=6) and tonsils (n=3).