Optofluidic Device For Ultra-sensitive Detection Of Proteins Using Surface-enhanced Raman Spectroscopy

摘要

An optofluidic device is reported in this paper that can highly improve the robustness of surface-enhanced Raman scattering (SERS) detection and provide fingerprint information of proteins with a concentration in the nano-gram per liter range within minutes. Moreover, the conformational change of protein can also be obtained using this device. Fabricated by standard photolithography processes, the optofluidic device has a step microfluidic-nanofluidic structure, which provides robust SERS detection. The sensitivity of the device is investigated using insulin and albumin as target analytes at a concentration of 0.9 ng/L. The ability to detect conformational changes of proteins using this technology is also shown by probing these analytes before and after their denaturation.