In situ formation of nano-hydroxyapatite whisker reinfoced porous β-TCP scaffolds

摘要

The preparation of porous ceramic scaffolds with nano-hydroxyapatite (HA) whiskers was reported in this work. By adding kalium ion and adjusting the pH value of solution, the thermal stability of nano-HA whiskers were improved significantly. The whiskers in the ceramic scaffolds can be kept completely after sintered at 1000 ℃. The nano-HA whisker was about 2 μm in length and 100 nm in diameter. The raw powders and scaffolds were observed by SEM and TEM, and the phase composition of samples was determined by XRD. The nHAw/β-TCP/BG scaffolds exhibited interconnected porous structures and enhanced mechanical properties as compared to those β-TCP/BC scaffolds. In order to assess the efforts of nano-HA whiskers on the behavior of osteoblasts cultured in vitro, the scaffolds were seeded with MC3T3-E1 and cultured for the same period. The cell viability and proliferation was assessed by the MTT assay and observed using laser scanning confocal microscopy. The Results suggest that the nano-HA whisker could be used to improve the cell adhesion and proliferation.