Fabrication of poly o-cresophthalein complexone film modified electrode and its application to simultaneous determination of purine bases in denatured DNA

摘要

A novel poly o-cresophthalein complexone film (POCF) modified electrode was fabricated and used as a sensor for the simultaneous detection of adenine and guanine. By comparing with the bare paraffin wax impregnated graphite electrode (PIGE), POCF modified electrode showed remarkable increase in the oxidation peak currents of adenine and guanine. The surface morphology of the POC polymer film and its nature on the PIGE were characterized by field emission scanning electron microscopy, X-ray photoelectron spectroscopy, Raman spectroscopy, cyclic voltammetry, electrochemical impedance spectroscopy and differential pulse voltammetry. The POCF modified electrode exhibited a linear range of 0.08 mu M-200 mu M with a low detection limit of 0.02 mu M (S/N) for adenine and guanine, respectively. Further, the proposed POCF modified electrode was used for simultaneous detection of adenine and guanine in denatured CT-DNA and moth DNA with satisfactory results. The value of (G + C)/(A + T) for CT-DNA and moth DNA was calculated to be 0.79 and 0.78 respectively. The prepared POCF modified electrode showed high reproducibility and excellent stability.