US Patent Provisional Application No. 61/932, filed Jan. 29, 2014, based on US Patent Act 119, paragraph (e). No. 935, US Provisional Application No. 61 / 878,769, filed September 17, 2013, and US Provisional Application No. 61 / 814,920, filed April 23, 2013. In addition, this application is a U.S. patent application filed simultaneously with the same applicant's co-pending applications YacubHANNA, NoaNOVERSHTERN, and YoachRAIS (name of invention 'isolated naive pluripotent stem cells and methods for generating them' It also relates to ISOLATEDNAIVEPLURIPOTENSTEMCELLSANDMETHODSOFGENERATINGSAME))) (agent case record book No. 58870). The contents of the above application are incorporated herein by reference in their entirety.

摘要

An isolated human naive pluripotent stem cell (PSC), wherein: (i) when the naive PSC is a female PSC, the naive female PSC is a non-specific gene for X inactivation specific transcript (XIST) An isolated human naïve PSC having two methylated alleles, and (ii) when the naive PSC is a male PSC, the naïve male PSC has one unmethylated allele of the XIST gene Is provided. Also, culture media, ERK1 / 2 inhibitor, GSK3beta inhibitor, p38 inhibitor, JNK inhibitor, STAT3 activator, and bFGF, TGFbeta1, PKC inhibitor, ROCK inhibitor, and NOTCH inhibitor Provided is a culture medium containing at least one drug selected from the group consisting of, or at least one drug selected from the group consisting of a TGFR inhibitor, FGFR inhibitor, PKC inhibitor, ROCK inhibitor, and NOTCH inhibitor Is done.