cDNA cloning and sequence analysis of genome segments S8 form rice black-streaked dwarf virus

摘要

Genome segments S8 of two Chinese isolates of rice black-streaked dwarf virus (RBSDV), one from Zhejiang Province and another form Hebei Province, were amplified by RT-PCR and se- quenced. Both segments consisted of 1936 nts in full length (EMBL accession numbers were AJ297431 and AJ297432, respectively) and contained only one big open reading frame which encoded a polypep- tide with molecular weight of 68kD. The tow Chinese isolates shared 94.0/100 and 96.5/100 identity at nucleotide and amino acid level, respectively. They shared 94.5-94.9/100 and 92.5-92.9/100 homolo- gy with S8 of RBSDV Japanese isolate at nucleotide and amino acid level, respectively; shared 85.1- 87.6/100 and 91.7-91.9/100 homology with S7 of Italian MRDV (maize rough dwarf virus).