Influence of CO_2 pneumoperitoneum on intracellular pH and signal transduction in cancer cells

摘要

Object: The authors studied the influence of CO_2 pneumoperitoneum on intracellular pH and signal transduction arising from cancer cell multiplication in laparoscopic tumor operation. Method: They set up a simulation of pneumoperitoneum under different CO_2 pressure, and then measured the variation of intracellular pH (pHi) at different time and the activity of protein kinase C (PKC) and protein phosphatase 2a (PP2a) at the end of the pneumoperitoneum. After 1 week, the concentration of cancer cells in the culture medium was calculated. Result: When the pressure of CO_2 pneumoperitoneum was 0, 10, 20, 30 mmHg respectively, the average pHi was 7.273, 7.075, 6.783, 6.693 at the end of the pneumoperitoneum; PKC activity was 159.4, 168.5, 178.0, 181.6 nmol/(g·min) and PP2a was 4.158.3, 4066.9, 3984.0, 3878.5 nmol/(g·min) respectively. After 1 week, the cancer cells concentration was 2.15x10~5, 2.03x10~5, 2.20x10~5, 2.18x10~5 L~(-1). Conclusion: CO_2 pneumoperitoneum could promote acidosis in cancer cells, inducing the activation of protein kinase C and deactivation of protein phosphatase 2a, but it could not accelerate the mitosis rate of the cancer cells.