A 240-Fold Electrostatic Rate-Enhancement for Acetylcholinesterase--Substrate Binding Can Be Predicted by the Potential within the Active Site

摘要

An approximate relation that allows easy prediction of klip, the enhancement of enzyme--substrate binding rates by an interaction potential U. was derived recently by one of us.' This is given by where the average of the Boltzmann factor is over the region in which the substrate can effectively bind to the enzyme. Equation 1 becomes exact when this binding region reduces to a point. In this paper, we present a test of this relation on the enzyme Torpedo californica acetylcholinesterase (TcACHE).