Replication of a DNA Microarray

摘要

Here we report a method for efficient replication of DNA microarrays.As shown in Scheme 1,the approach comprises three steps.First,a master DNA microarray consisting of single-stranded DNA(ssDNA)elements is exposed to a solution containing the biotin-functionalized complement of each array element.Following hybridization,a replica surface modified with streptavidin is brought into contact with the master.This results in linking of the biotin-functionalized complement with the replica surface.Next,the replica is separated from the master,and some or all of the complementary strands are transferred to the replica surface.The resulting complementary DNA microarray now contains position-coded sequences that mirror the information contained on the master DNA microarray.Finally,ssDNA on the master can be rehybridized and the procedure repeated to produce additional replicas.