Protein Assembly Line Components in Prodigiosin Biosynthesis:Characterization of PigA,G,H,I,J

摘要

The tripyrrolic products of the prodiginine class of natural products are signature antibiotics of Serratia and Streptomyces bacterial strains and have a plethora of activities in multicellular eukaryotes.Prodigiosin,the red pigment produced by Serratia marcescens,has a prototypic scaffold with the A and B pyrrole rings connected directly as a bipyrrole unit,while the B and C rings have the methylene bridging connectivity typically found in heme-type pigments of life.Prodiginine biosynthetic gene clusters from Streptomyces coelicolor and from Serratia marcescens have been sequenced and bioinformatics-based pathways of tripyrrole assembly proposed.Each of the three pyrrole rings is proposed to arise from a different amino acid,ring A from L-proline,ring B in part from L-serine,and ring C in part from glycine.