Immune Responses in Mice Injected with gD Plasmid DNA of Infectious Bovine Rhinotracheitis Virus

摘要

The gene encoding gD of isolate Luojing of infectious bovine rhinotracheitis virus (IBRV)was amplified, sequenced and cloned into plasmid pcDNA 3.1, resulting in a recombinant pcDNA-gD. Groups of BALB/c mice were injected with 100 mu g of plasmid onlyor together with liposome. After immunization serum samples were collected from mice every 2 weeks for a 10-week period and tested for protein-specific antibody with enzyme-linked im-munosorbent assay (EUSA). It was showed that the plasmid encoding IBRVglycopretein D developed gene-specific antibody. This report indicates the potential of DNA injection as a method of vaccination.